A Study of Sperm Quality Characteristics Changes in Different Storage Temperatures above Freezing Point

Introduction: Sperm is very sensitive to temperatures changes as it is capable of changing the sperm characteristics. Therefore, the aim of this study was to identify the optimum storage temperature above freezing point for sperm transportation in order to maintain the good sperm characteristics.

Objective: Sperm characteristics such as motility, viability and morphology were evaluated after 1, 2 and 3 hour(s) stored at different temperatures (4?C, 25?C and 37?C).

Method: The fresh semen bovine were collected using artificial vagina at Institut Biodiversiti Veterinar Kebangsaan, Jerantut (Pahang). Sperm motility and viability were carried out using computer-assisted sperm analysis (CASA) while sperm morphology was carried out using scanning electron microscope (SEM).

Result: The results obtained demonstrated that 25°C has high sperm progressive movement as well as sperm viability compared to 4°C and 37°C. Sperm stored at 25°C showed normal morphological structure whereas there were morphological alteration at storage temperature of 4°C and 37°C.

Conclusion: In conclusion, 25?C is the best storage temperature above freezing point to maintain sperm characteristics during sperm transportation.

Author(s): Jamaludin Mohamed, Maizatul Nadhirah Ismail, Tham Yu Chou, Santhana Raj Louis, Siti Balkis Budin

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